Cd-induced changes in leaf proteome of the hyperaccumulator plant Phytolacca americana

作  者:Le Zhao, Yong-Le Sun, Su-Xia Cui, Mei Chen, Hao-Meng Yang, Hui-Min Liu, Tuan-Yao Chai, Fang Huang
影响因子:3.155
刊物名称:Chemosphere
出版年份:Sep-2011
卷:85  期:1  页码:56-66

论文摘要:

 Cadmium (Cd) is highly toxic to all organisms. Soil contamination by Cd has become an increasing problem
worldwide due to the intensive use of Cd-containing phosphate fertilizers and industrial zinc mining.
Phytolacca americana L. is a Cd hyperaccumulator plant that can grow in Cd-polluted areas. However, the
molecular basis for its remarkable Cd resistance is not known. In this study, the effects of Cd exposure on
protein expression patterns in P. americana was investigated by 2-dimensional gel electrophoresis (2-DE).
2-DE profiles of leaf proteins from both control and Cd-treated (400 lM, 48 h) seedlings were compared
quantitatively using ImageMaster software. In total, 32 differentially expressed protein spots were identified
using MALDI-TOF/TOF mass spectrometry coupled to protein database search, corresponding to 25
unique gene products. Of those 14 were enhanced/induced while 11 reduced under Cd treatment. The
alteration pattern of protein expression was verified for several key proteins involved in distinct metabolic
pathways by immuno-blot analysis. Major changes were found for the proteins involved in photosynthetic
pathways as well as in the sulfur- and GSH-related metabolisms. One-third of the up-regulated
proteins were attributed to transcription, translation and molecular chaperones including a protein
belonging to the calreticulin family. Other proteins include antioxidative enzymes such as 2-cys-peroxidase
and oxidoreductases. The results of this proteomic analysis provide the first and primary information
regarding the molecular basis of Cd hypertolerance in P. americana.