An aquaporin PvTIP4;1 from Pteris vittata may mediate arsenite uptake

作  者:He ZY, Yan HL, Chen YS, Shen HL, Xu WX, Zhang HY, Shi L, Zhu YG*, Ma M*
影响因子:7.672
刊物名称:New Phytologist
出版年份:2016
卷:209  期:2  页码:746-761

论文摘要:

  • The fern Pteris vittata is an arsenic hyperaccumulator. The genes involved in arsenite (As(III)) transport are not yet clear. Here, we describe the isolation and characterization of a new P. vittata aquaporin gene, PvTIP4;1, which may mediate As(III) uptake.
  • PvTIP4;1 was identified from yeast functional complement cDNA library ofP. vittata. Arsenic toxicity and accumulating activities of PvTIP4;1 were analyzed in Saccharomyces cerevisiae and Arabidopsis. Subcellular localization of PvTIP4;1–GFP fusion protein in P. vittata protoplast and callus was conducted. The tissue expression of PvTIP4;1 was investigated by quantitative real-time PCR. Site-directed mutagenesis of the PvTIP4;1 aromatic/arginine (Ar/R) domain was studied.
  • Heterologous expression in yeast demonstrates that PvTIP4;1 was able to facilitate As(III) diffusion. Transgenic Arabidopsis showed that PvTIP4;1 increases arsenic accumulation and induces arsenic sensitivity. Images and FM4-64 staining suggest that PvTIP4;1 localizes to the plasma membrane in P. vittatacells. A tissue location study shows that PvTIP4;1 transcripts are mainly expressed in roots. Site-directed mutation in yeast further proved that the cysteine at the LE1 position of PvTIP4;1 Ar/R domain is a functional site.
  • PvTIP4;1 is a new represented tonoplast intrinsic protein (TIP) aquaporin fromP. vittata and the function and location results imply that PvTIP4;1 may be involved in As(III) uptake.