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沈世华


职务/职称: 研究员
联系电话: (86)-010-62836545
电子邮件: shshen@ibcas.ac.cn
个人网页: http://klpr.ibcas.ac.cn/content/?155.html
课 题 组: 植物逆境信号应答分子机制研究组

沈世华,男,博士,研究员,博士生导师。

        1962年11月出生于重庆市綦江县,1983年在西南农业大学(现西南大学)获农学学士学位,1989年和1994年在中科院植物所获理学硕士和博士学位。1996年至1998年在日本筑波大学基因研究中心(Gene Research Center, University of Tsukuba, Japan.)从事博士后研究,1999年至2011年在日本国立农业科学研究所(National Institute of Agrobiological Sciences, Japan)进行访问研究。兼任中科院唐山高新技术研发与转化中心工程植物事业部部长,中国植物生理学会植物生物质与生物能源专业委员会委员。2002年回国以来,申报专利10项,发表论文60余篇,SCI收录刊物论文50余篇。其中,第一作者和通讯作者论文47篇。已培养硕士和博士20名;正在指导博士后2名、博士生和硕士生10名。

主要研究工作:

以重要资源植物为研究对象,采用植物生理学、分子生物学和蛋白质组学等研究手段,探索植物逆境适应机制,揭示植物感应、传递、应答胁迫环境信号的途径,寻找网络调控响应过程中的重要关键基因,通过现代先进生物学技术进行分子改良,提高植物抗逆性和防御能力,为生态修复、环境治理、绿色高效农业提供理论依据和技术保障。

1. 植物逆境蛋白质组学研究,如水稻响应干旱、低温、盐碱、机械伤害,麻疯树应答低温,菊芋响应干旱、盐碱蛋,小泥碗癣脱水、低温胁迫等蛋白质组学解析。

2. 重要关键抗逆基因分离及其功能研究,从资源植物麻疯树、构树等中分离克隆到20多个与逆境应答和生长发育相关的重要关键基因,如调控耐干旱、低温、盐碱等逆境基因表达的转录因子基因DREBDOFMYBNAC等,并对水稻、麻疯树、构树等进行转基因研究。

3. 构树、麻疯树等资源植物产业化关键技术研发与示范推广等,其中,杂交构树已在全国20多个省市区边际土地上示范种植,在干旱、盐碱、沙荒地、矿山生态修复等方面具有广阔的应用前景。

主持和参加的科研项目:

在研课题:

[1]     双流冬草莓脱毒复壮及新品种示范推广, 院地合作项目(批准号:XBCD-2011-009)(2011.01-2013.12)资助金额22万;主持人

[2]     耐盐植物筛选繁育与适应性种植滨海盐碱地绿化技术集成与示范,院重点项目(编号:KZCX2-YW-359)资助金额120万,共同支持

[3]     麻疯树耐寒转录因子基因克隆及其功能研究,国家基金委面上项目(批准号:31270653)(2013.01~2016.12),主持人

[4]      “生物固氮作用的分子机理研究”,科技部“973”计划(批准号:2010CB1265)(2010.01~2014.12),“水稻与根瘤菌互作用机制”子课题,资助金额200万元;主持人:沈世华。

结题课题:

[5]      “水稻抗寒性状形成重要关键基因克隆及其功能验证”,科技部其它项目(批准号:2009ZX08009-074B)(2009.06~2011.12),资助金额120万元;主持人:沈世华。

[6]      “麻疯树油脂合成机制与分子改良研究” 国家自然基金重点项目(批准号:U0733005)(2008.01~2011.12),“麻疯树种子油脂合成蛋白质组学解析”课题,资助金额31万元;主持人:沈世华。

[7]      “滨海耐盐构树选育与种植试验示范”,中科院支持天津建设科技行动计划项目(批准号:TJXZ2-YW-25)(2009.01~2010.12),资助金额50万元;主持人:沈世华。

[8]      “麻疯树、木薯重要性状形成机制及新种质创”,中科院知识创新工程重要方向项目(批准号:KSCX2-YW-G-035)(2008.09~2010.12),“麻疯树抗性机制”课题,资助金额50万元;主持人:沈世华。

[9]      “水稻灌浆期抗低温性状形成的代谢途径解析”,科技部“863”项目(批准号:2007AA10Z109)(2007.10~2010.08),资助金额78万元;主持人:沈世华。

[10]  “枯草芽孢杆菌KB-1111以及新型植物生防与促生剂生产技术的引进”,农业部“948”项目(批准号:2004-Z26)(2004.10~2007.12),资助金额60万元;主持人:沈世华。

[11]  “水稻应答赤霉素的蛋白质组学研究”,国家自然基金面上项目(批准号:30470168)(2005.01~2007.12),资助金额23万元;主持人:沈世华。

[12]  “高效生物固氮作用机理及其在农业中的应用”,科技部“973”项目(批准号:G2001CB108902)(2002.01~2006.08),“我国特有苜蓿根瘤菌和其宿主植物苜蓿的共生固氮功能基因组”课题,资助金额40万元;主持人:沈世华。

[13]  “主要农作物DREB基因的克隆及转化”,科技部“863”项目(批准号:2002AA224091)(2002.01~2005.12),“DREB基因克隆及其功能研究”子课题,资助金额25万元;主持人:沈世华。

研究论文(注*为通讯作者):

2012

Ji KX, Wang YY, Sun WN, Lou QJ, Mei HW, Shen SH, Chen H*. 2012. Drought-responsive mechanisms in rice genotypes with contrasting drought tolerance during reproductive stage. Journal of Plant Physiology, 169: 336-344

2011

Chen Q, Zhang MD, Shen SH*. 2011. Effect of salt on malondialdehyde and antioxidant enzymes in seedling roots of Jerusalem artichoke (Helianthus tuberosus L.). Acta Physiologiae Plantarum, 33: 273-278.

Zhang MD, Chen Q, Shen SH*. 2011. Physiological responses of two Jerusalem artichoke cultivars to drought stress induced by polyethylene glycol. Acta Physiologiae Plantarum, 33: 313-318.

Fan WH, Cui WT, Li XF, Chen SY, Liu GS*, Shen SH*. 2011. Proteomics analysis of rice seedling responses to ovine saliva. Journal of Plant Physiology, 68: 500-509.

Peng XJ, Ma XY, Fan WH, Su M, Cheng LQ, Iftekhar A, Lee BH, Qi DM, Shen SH*, Liu GS*. 2011. Improved drought and salt tolerance of Arabidopsis thaliana by transgenic expression of a novel DREB gene from Leymus chinensis. Plant Cell Reports, 30: 1493-1502.

He DL, Han C, Yao JL, Shen SH and Yang PF*. 2011. Constructing the metabolic and regulatory pathways in germinating rice seeds through proteomic approach. Proteomics, 11:  2693–2713.

Chen Q, Zhang MD, Shen SH*. 2011. Comparison of protein extraction methods suitable for proteomics analysis in seedling roots of Jerusalem artichoke under salt (NaCl) stress. African Journal of Biotechnology, 10: 7650-7657.

Yang J, Yang MF, Zhang WP, Chen F, Shen SH*. 2011. A putative flowering-time-related Dof transcription factor gene, JcDof3, is controlled by the circadian clock in Jatropha curcas. Plant Science, 181: 667-674.

Liu H, Yang ZL, Yang MF, Shen SH*. 2011. The differential proteome of endosperm and embryo from mature seed of Jatropha curcas. Plant Science, 181: 660-666.

Tang MJ, Liu XF, Deng HP*, Shen SH*. 2011. Over-expression of JcDREB, a putative AP2/EREBP domain-containing transcription factor gene in woody biodiesel plant Jatropha curcas, enhances salt and freezing tolerance in transgenic Arabidopsis thalia. Plant Science, 181: 623-631.

Li XJ, Yang MF, Zhu Y, Liang Y, Shen SH*. 2011. Proteomic analysis of salt stress responses in rice shoot. Journal of Plant Biology, 54: 384-395

2010年以前

Liu Y, Chen H, Zhuang DF, Jiang D, Liu J, Wu GJ, Yang MF*, Shen SH*. 2010. Characterization of a DRE-binding transcription factor from asparagus (Asparagus officinalis L.), and its overexpression in Arabidopsis resulting in salt- and drought-resistant transgenic plants. International Journal of Plant Sciences, 171: 12-23.

Ji KX, Chi F, Yang MF, Shen SH*, Jing YX*, Cheng HP and Dazzo FB. 2010. Movement of Rhizobia inside tobacco and lifestyle alternation from endophytes to free-living Rhizobia on leaves. Journal of Microbiology and Biotechnology, 20: 238-244.

Li XJ, Yang MF, Chen H, Qu LQ, Chen F, Shen SH*. 2010. Abscisic acid pretreatment enhances salt tolerance of rice seedlings: Proteomic evidence. BBA-Proteins and Proteomics, 1804: 929-940.

Chi F, Yang PF, Han F, Jing YX*, Shen SH*. 2010. Proteomic analysis of rice seedlings infected by Sinorhizobium meliloti 1021. Proteomics, 10: 1861-1874.

Yang J, Yang MF, Wang D, Chen F* and Shen SH*. 2010. JcDof1, a Dof transcription factor gene, is associated with the light-mediated circadian clock in Jatropha curcas. Physiologia Plantarum, 139: 324-334.

Pan XL*, Jiang QY, Pan QY, Wen XF, Shi YD, Wang YJ, Pan TY, Xie SG, Zhang GY, Wu SJ, Chai YF, Zhang C, Wu ZX and Shen SH*. 2009. Proteomic analysis of ‘hybrid necrosis’ in wheat (Triticum aestivum) leaves. Functional Plant Biology, 36: 251–259.

Zhang CX, Zhao X, Han F, Yang MF*, Jing YX, Chida T, Shen SH*. 2009. Comparative proteome analysis of two antagonist Bacillus subtilis strains. Journal of Microbiology and Biotechnology, 19: 351-358.

Yang MF, Liu YJ, Liu Y, Chen H*, Chen F, Shen SH*. 2009. Proteomic analysis of oil mobilization in seed germination and postgermination development of Jatropha curcas. Journal of Proteome Research, 8: 1441-1451.

Wang XQ, Yang PF, Liu Z, Liu WH, Hu Y, Chen H, Kuang TY, Pei ZM, Shen SH, He YK*. 2009. Exploring the mechanism of Physcomitrella patens desiccation tolerance through a proteomic strategy. Plant Physiology, 149: 1739-1750.

Liu H, Liu YJ, Yang MF*, Shen SH*. 2009. A Comparative analysis of embryo and endosperm proteome from seeds of Jatropha curcas. Journal of Integrative Plant Biology, 51: 850-857.

[31] Wang XQ, Yang PF, Liu Z, Xu Y, Kuang TY, Shen SH*, He YK*. 2009. Proteomic analysis of the cold stress response in the moss, Physcomitrella patens. Proteomics, 9: 4529-4538.

Han F, Chen H, Li XJ, Yang MF*, Liu Ge*, Shen SH*. 2009. A comparative proteomic analysis of rice seedlings under various high-temperature stresses. BBA-Proteins and Proteomics, 1794: 1625-1634.

Zhang CX, Zhao X, Jing YX, Chida T, Chen H, Shen SH*. 2008. Phenotypic and biological properties of two antagonist Bacillus subtilis strains. World Journal of Microbiology Biotechnology, 24: 2179-2181.

Wang XQ, Yang PF, Gao Q, Liu XL, Kuang TY, Shen SH*, He YK*. 2008. Proteomic analysis of the response to high-salinity stress in Physcomitrella patens, 228: 167-177.

Jiang QY, Chen H, Pan XL*, Pan QY, Shi YH, Li XR, Zhang GY, Wang YJ, Xie SG, Shen SH*. 2008. Proteomic analysis of wheat (T. aestivum L) hybrid necrosis. Plant Science, 175: 394-403

Lu P, Chen LJ, Li GX, Shen SH, Wang LL, Jiang QY*, Zhang JF. 2007. Influence of furfural concentration on growth and ethanol yield of Saccharomyces kluyveri. Journal of Environmental Sciences.19: 1528-1532.

Yang PF, Chen H, Liang Y, Shen SH*. 2007. Proteomic analysis of de-etiolated rice seedlings upon exposure to light. Proteomics, 7: 2459-2468.

Yang PF, Li XJ, Wang XQ, Chen H, Chen F, Shen SH*. 2007. Proteomic analysis of rice (Oryza sativa) seeds during germination. Proteomics, 7: 3358-3368.

Liang Y, Chen H, Tang MJ and Shen SH*. 2007. Proteome analysis of an ectomycorrhizal fungus Boletus edulis under salt shock. Mycological Research, 111: 939-946.

Liang Y, Chen H, Tang MJ, Yang PF, Shen SH*. 2007. Responses of Jatropha curcas seedlings to cold stress: photosynthesis related proteins and chlorophyll fluorescence characteristics. Physiologia Plantarum, 131: 508-517.

Tang MJ, Sun JW, Liu Y, Chen F, Shen SH*. 2007. Isolation and functional characterization of the JcERF gene, a putative AP2/EREBP domain-containing transcription factor, in woody oil plant Jatropha curcas. Plant Molecular Biology, 63: 419-428.

Han WW, Shen SH*, Tai PD. 2007. Proteome analysis of inhibitory effect of gadolinium on Sinorhizobium fredii. Journal of Rare Earths, 25: 106-110.

Yang PF, Shen SH*, Kuang TY. 2006. A comparative analysis of the endosperm proteins separated by two-dimensional electrophoresis for two cultivars of hybrid rice (Oryza sativa L.). Journal of Integrative Plant Biology, 48: 1028-1033.

Zuo P, Chen H*, Wu YS, Shen SH, Wang P, Ai XC, Zhang JP*, Li LB, Kuang TY. 2006. Effects of different aggregation forms of LHC II from Bryopsis corticulans on the excited state properties of Chl a. Chinese Science Bulletin, 51: 1444-1451.

Zhou XJ, Liang Y, Chen H, Shen SH*, Jing YX*. 2006. Effects of rhizobia inoculation and nitrogen fertilization on soybean photosynthetic physiology. Photosynthetica, 44: 530-535.

Chen YM, Chen T, Shen SH, Zheng MZ, Guo YM, Lin JX*, Baluska F, Samaj J. 2006. Differential display proteomic analysis of Picea meyeri pollen germination and pollen tube growth after actin depolymerization by latrunculin B. The Plant Journal, 47: 174-195.

Yang PF, Liang Y, Shen SH*, Kuang TY. 2006. Proteome analysis of rice uppermost internodes at the milky stage. Proteomics, 6: 3330-3338.

Yang PF, Li XJ, Liang Y, Jing YX, Shen SH*, Kuang TY. 2006. Proteomic analysis of the response of Liangyoupeijiu (A super high-yield hybrid rice) seedlings to cold stress. Journal of Integrative Plant Biology, 48: 945-951.

Lü SY, Jing YX, Shen SH, Zhao HY, Ma LQ, Zhou XJ, Ren Q,Li YF*. 2005. Antiporter Gene from Hordum brevisubulatum (Trin.) Link and Its Overexpression in Transgenic Tobaccos. Journal of Integrative Plant Biology, 47: 343-349.

Chi F, Shen SH, Cheng HP, Jing YX*, Yanni YG. and Dazzo FB. 2005. Ascending migration of endophytic Rhizobia from roots to leaves inside rice plants and assessment of their benefits to the growth physiology of rice. Applied and Environmental Microbiology, 71: 7271-7278.

Chen H*, Shen SH, Liang Y, Leng J, Tang MJ, Gong YD. 2005. Evidence for dissociation of chlorophyll b from the main light-harvesting complex in the oligomerization state isolated from marine alga, Bryopsis corticulans. Biochimica et Biophysica Acta- Bioenergetics, 1707: 170-179.

Tang MJ,Lü SY, Jing YX, Zhou XJ, Sun JW, Shen SH*. 2005. Isolation and identification of a cold-inducible gene encoding a putative DRE-binding transcription factor from Festuca arundinacea. Plant Physiology and Biochemistry, 43: 233-239.

Qi SW, Yang PF, Jing YX, Shen SH* & Yang SS*. 2004. A proteomic analysis of bacterial strain Sinorhizobium fredii RT19 subjected to salt shock. Chinese Science Bulletin, 49: 1828-1833.

Chen H*, Shen SH, He JF, Leng J, Li LB & Kuang TY. 2004. A light-harvesting siphonaxanthin- chlorophyll a/b-protein complex of marine green alga, Bryopsis corticulans. Chinese Science Bulletin, 49: 1936-1941.

Yang GX, Jan A, Shen SH, Yazaki J, Ishikawa M, Shimatani Z, Kishimoto N, Kikuchi S, Matsumoto H and Komatsu S*. 2004. Microarray analysis of brassinosteroids- and gibberellin-regulated gene expression in rice seedlings. Molecular Genetics and Genomics, 271:468-478.

Shen SH and Jing YX*. 2003. Present status and development on biological nitrogen fixation research in China. Chinese Science Bulletin, 48: 954-960.

Yang GX, Shen SH, Yang SH, Komatsu S*. 2003. OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced in response to cold and gibberellin. Plant Physiology and Biochemistry, 41: 369-374.

Shen SH*, Jing YX, Kuang TY. 2003. Proteomics approach to identify wound-response related proteins from rice leaf sheath. Proteomics, 3: 527-535.

Shen SH, Sharma A, Komatsu S*. 2003. Characterization of Proteins Responsive to Gibberellin in the Leaf-Sheath of Rice (Oryza sativa L.) Seedling Using Proteome Analysis. Biological Pharmaceutical Bulletin, 26: 129-136.

Komatsu S*, Konishi H, Shen SH, Yang GX. 2003. Rice proteomics: a step toward functional analysis of the rice genome. Molecular Cellular & Proteomics, 2: 2-10.

Shen SH, Matsubae M, Takao T, Komatsu S*. 2002. A proteomics analysis of leaf sheath from rice. Journal of Biochemistry, 132: 613-620.

专利:

[1] 沈世华、孙静文、刘  杰等。一种植物DREB转录因子及其编码基因与应用。国家发明专利,ZL200510082838.0。2007-01-24。

[2] 沈世华、唐明娟、刘  杰。一个麻疯树冷诱导转录因子及其编码基因与应用。国家发明专利,ZL200610000259.1。2007-12-26。

[3] 唐明娟、沈世华、刘  杰。一个麻疯树盐诱导基因与应用。国家发明专利,ZL200610000100.X。2010-02-03。

[4] 沈世华、刘玉君、苟  平。一种麻疯树种子胚乳蛋白质提取方法。国家发明专利,CN101171952。2008-05-07。

[5] 沈世华、刘玉君、苟  平。麻疯树种子胚乳蛋白质高分辨率的分离方法。国家发明专利,CN101173904A。2008-05-07。

[6] 沈世华、刘玉君、苟  平。一种麻疯树种子油体蛋白质提取方法。国家发明专利,CN101418034。2009-04-29。

[7] 沈世华、刘玉君、苟  平。一种麻疯树种子油体蛋白质分离方法。国家发明专利,CN101343312。2009-01-14。

[8] 沈世华、刘  杰、刘  蕴等。一种植物抗逆相关蛋白及其编码基因与应用。国家发明专利,CN101250220A。2008-08-27。

[9] 李国学、路鹏、沈世华等。一种利用秸秆水解物发酵生产乙醇的微生物菌剂及其应用。国家发明专利,CN101381680。2009-03-11。

[10] 刘公社、沈世华、彭献军等。一种羊草干旱诱导转录因子及其编码基因与应用。国家发明专利,CN101684149。2010-03-31。

获奖及荣誉:

1. 2008年,“杂交构树工厂化快繁育苗技术”获大连市科技进步三等奖(获奖证书号:2008J-3-34-01)。


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