姓  名: 王台
职务/职称: 研究员、研究组组长
联系电话: (86)-010-62836210
电子邮件: twang@ibcas.ac.cn
个人网页: http://www.klpmp.ibcas.ac.cn/kydw/zzfy/wt/202101/t20210111_615173.html
课 题 组: 花粉与种子发育研究组
王台,男,博士,研究员,博士生导师。

研究员,博士研究生导师。1985在河南师范大学获学士学位,1988年在武汉大学获硕士学位,1997年在中科院植物所获博士学位。19931994 年东京大学UNESCO Fellow19971998年日本原子能研究所植物科学研究中心STA Fellow。现任中科院植物分子生理学重点实验室主任,中科院植物所学术委员会常委、学位委员会委员,中国科学院大学岗位教授,中国植物学会理事会常务理事,中国植物生理与分子生物学学会细胞与发育生物学专业委员会副主任,全国专业标准化技术委员会委员,《植物学报》主编。先后担任中国科学院战略性科技先导专项《分子模块设计育种创新体系》和《种子精准设计与创造》总体组成员。曾任国家植物基因研究中心(北京)项目科学家,中国植物学会副秘书长,中国细胞生物学会染色体基因组蛋白质组分会副会长,中国生物化学与分子生物学会蛋白组学专业委员会委员, Frontier in Biology 副主编,Asia Coordinator for International Plant Proteomics Organization (INPPO)等。2012年获国务院政府特殊津贴

主要研究工作:

1.花粉细胞命运决定与发育的分子生理机制

以突变体为材料,综合利用分子遗传学、细胞生物学、生理学技术手段,结合蛋白质组、基因组等组学数据分析,解析花粉细胞命运决定与发育的调控机制与分子网络,为作物雄性不育系创制和杂种优势利用提供分子元件以及理论与技术。

2.作物种子发育及优质性状形成的分子基础

利用从水稻种质资源中筛选的优异种质资源材料,通过遗传群体构建、表型鉴定及全基因组关联分析和数量性状位点定位,解析水稻籽粒灌浆、成熟、加工与外观品质性状的调控因子和分子网络,阐释灌浆与成熟期温度等环境因子影响品质性状的机制。同时,也启动了重要饲草苜蓿高产优质分子模块解析及高产优质性状的分子设计等研究。

研究论文(注*为通讯作者):

2022

Feng YX, Wang T, Liu LT*. 2022. An efficient clearing protocol for the study of seed development in Tomato (Solanum lycopersicum L.). Jove-Journal of Visualized Experiments, 187:e64445, doi:10.3791/64445.

Deng ZY#, Liu YX#, Gong CY, Chen BT, Wang T*. 2022. Waxy is an important factor for grain fissure resistance and head rice yield as revealed by a genome-wide association study. Journal of Experimental Botany, 73:6942-6954. 

Song YY, Tang YY, Liu LT, Xu YY, Wang T*. 2022. The methyl-CpG-binding domain family member PEM1 isessential for Ubisch body formation and pollen exine development in rice. Plant Journal, 111:1283-1295.

2021

Liu LT*, Wang T*. 2021. Male gametophyte development in flowering plants: A story of quarantine and sacrifice. Journal of Plant Physiology, 258: 153365. 

Lu YL, Song YY, Liu LT*, Wang T*. 2021. DNA methylation dynamics of sperm cell lineage development in tomato. Plant Journal, 105: 565-579.

2020

Yang F, Liu LT, Wang T*. 2020. Pollen germination is impaired by disruption of a Shaker K+ channel OsAKT1.2 in rice. Journal of Plant Physiology, 248:153140

2018

Liu LT, Lu YL, Wei LQ, Yu H, Cao YH, Li Y, Yang N, Song YY, Liang CZ, Wang T*. 2018. Transcriptomics analyses reveal the molecular roadmap and long non-coding RNA landscape of sperm cell lineage development. Plant J., 96: 421-437.

Han B, Yang N, Pu H, Wang T*. 2018. Quantitative proteomics and cytology of rice pollen sterol-rich membrane domains reveals pre-established cell polarity cues in mature pollen. J. Proteome Res., 17: 1532-1546.

2017

Yang N, Wang T*. 2017. Comparative proteomic analysis reveals a dynamic pollen plasma membrane protein map and the membrane landscape of receptor-like kinases and transporters important for pollen tube growth and interaction with pistils in rice. BMC Plant Biol., 17: 2.

Yang N, Han B, Wang T*. 2017. Protein isolation from plasma membrane, digestion and processing for strong cation exchange fractionation. Bio-protocol, 7: e2298.

Yang N, Han B, Liu L, Yang H, Wang T*. 2017. Plasma membrane preparation from Lilium davidii and Oryza sativa mature and germinated pollen. Bio-protocol, 7: e2297

2016 

Liu LT, Zheng CH, Kuang BJ, Wei LQ, Yan LF, Wang T. 2016. Receptor-Like kinase RUPO interacts with potassium transporters to regulate pollen tube growth and integrity in rice. PLoS Genetics, doi:10.1371/journal.pgen.1006085

Yu HT, Wang T. 2016. Proteomic dissection of endosperm starch granule associated proteins reveals a network coordinating starch biosynthesis and amino acid metabolism and glycolysis in rice endosperms. Frontiers in Plant Science, doi: 10.3389/fpls.2016.00707

Li Q, Deng ZY, Gong CY, Wang T. 2016. The rice eukaryotic translation initiation factor 3 subunit f (OseIF3f) is involved in microgametogenesis. Frontiers in Plant Science, doi: 10.3389/fpls.2016.00532

Yang H, Yang N, Wang T. 2016. Proteomic analysis reveals the differential histone programs between male germline cells and vegetative cells in Lilium davidii. Plant Journal, 85: 660-674

2015

Lu YL, Wei LQ, Wang T. 2015. Methods to isolate a large amount of generative cells, sperm cells and vegetative nuclei from tomato pollen for “omics” analysis. Frontiers in Plant Science, doi: 10.3389/fpls.2015.00391

Deng ZY*, Liu LT*, Li T, Yan S, Kuang BJ, Huang SJ, Yan CJ, Wang T. 2015. OsKinesin-13A is an active microtubule depolymerase involved in glume length regulation via affecting cell elongation. Scientific Reports, 5: 9457 (*Co-authors)

2014

Wang Zizhang, Xue Zhen, Wang Tai*. 2014. Differential analysis of proteomes and metabolomes reveals additively balanced networking for metabolism in maize heterosis. Journal of Proteome Research, 13(9): 3987-4001.

2013

Zhao X, Yang N and Wang T. 2013. Comparative proteomic analysis of generative and sperm cells reveals molecular characteristics associated with sperm development and function specialization. Journal of Proteome Research, 12: 5058-5078

Zhang X, Wei LQ, Wang ZZ and Wang T. 2013. Physiological and molecular features of puccinellia tenuiflora tolerating salt and alkaline-salt stress. Journal of Integrative Plant Biology, 55: 262–276 

Deng ZY, Gong CY and Wang T. 2013. Use of proteomics to understand seed development in rice. Proteomics, 13: 1784–1800

Agrawal GK, Job D, Kieselbach T, Barkla BJ, Chen SX, Deswal R, Luthje S, Amalraj RS, Tanou G, Ndimba BK, Cramer R, Weckwerth W, Wienkoop S, Dunn MJ, Kim ST, Fukao Y, Yonekura M, Zolla L, Rohila JS, Waditee-Sirisattha R, Mas A, Wang T, Sarkar A, Agrawal R, Renaut J and Rakwal R. 2013. INPPO Actions and recognition as a driving force for progress in plant proteomics: change of guard, INPPO update, and upcoming activities. Proteomics, 13: 3093–3100 

Agrawal GK, Sarkar A, Righetti PG, Pedreschi R, Carpentier S, Wang T, Barkla B J, Kohli A, Ndimba BK, Bykova NV, Rampitsch C, Zolla L, Rafudeen MS, Cramer R, Bindschedler LV, Tsakirpaloglou N, Roya JN, Farrant JM, Renaut J, Job D, Kikuchi S and Rakwal R. 2013. A decade of plant proteomics and mass spectrometry:Translation of technical advancements to food security and safety issues. Mass Spectrometry Reviews, doi: 10.1002/mas

2012

Gong CY, Li Q, Yu HT, Wang ZZ and Wang T. 2012. Proteomics insight into the biological safety of transgenic modification of rice as compared with conventional genetic breeding and spontaneous genotypic variation. Journal of Proteome Research, 11, 3019-3029

Zhang H, Han B, Wang T, Chen SX, Li HY, Zhang YH and Dai SJ. 2012. Mechanisms of plant salt response: insights from proteomics. Journal of Proteome Research, 11, 49-67 

Yu HT, Xu SB, Zheng CH and Wang T. 2012. Comparative proteomic study reveals the involvement of diurnal cycle in cell division, enlargement, and starch accumulation in developing endosperm of Oryza sativa. Journal of Proteome Research, 11, 359–371 

Wang ZZ, Yan SJ, Liu CM, Chen F and Wang T. 2012. Proteomic Analysis reveals an aflatoxin-triggered immune response in cotyledons of arachis hypogaea infected with Aspergillus flavus. Journal of Proteome Research, 11, 2739-2753 

2011

Wei LQ, Yan LF and Wang T. 2011. Deep sequencing on genome-wide scale reveals the unique composition and expression patterns of microRNAs in developing pollen of Oryza sativa. Genome Biology,12, R53

An XJ, Deng ZY and Wang T. 2011. OsSpo11-4, a rice homologue of the archaeal TopVIA protein, mediates double-strand DNA cleavage and interacts with OsTopVIB. PLoS One, 6, e20327 

Yu JJ, Chen SX, Zhao Q, Wang T, Yang CP, Diaz C, Sun GR and Dai CJ. 2011. Physiological and proteomic analysis of salinity tolerance in puccinellia tenuiflora. Journal of Proteome Research, 10, 3852–3870 

Xu W, Yang R, Li M, Xing Z, Yang W, Chen G, Guo H, Gong X, Du Z, Zhang Z, Hu X, Wang D, Qian Q, Wang T, Su Z and Xue Y. 2011. Transcriptome phase distribution analysis reveals diurnal regulated biological processes and key pathways in rice flag leaves and seedling leaves. PloS One, 6, e17613

Wang ZZ and Wang T. 2011. Dynamic proteomic analysis reveals diurnal homeostasis of key pathways in rice leaves. Proteomics, 11:225-238

2010及以前

Han B, Chen S, Dai SJ, Yang N and Wang T. 2010. Isobaric Tags for Relative and Absolute Quantificationbased Comparative Proteomics Reveals the Features of Plasma Membrane-Associated Proteomes of Pollen Grains and Pollen Tubes from Lilium davidii. Journal of Integrative Plant Biology, 52 : 1043–1058 

Xu SB, Yu HT, Yan LF and Wang T. 2010. Integrated proteomic and cytological study of rice endosperms at the storage phase. Journal of Proteome Research, 9:4906-4918

Wei LQ, Xu WY, Deng ZY, Su Zhen, Xue Y and Wang T. 2010. Genome-scale analysis and comparison of gene expression profiles in developing and germinated pollen in Oryza sativa. BMC Genomics, 11:338

Zhao LF Hu YB, Chong K and Wang T. 2010. ARAG1, an ABA-responsive DREB gene, plays a role in seed germination and drought tolerance of rice. Annals of Botany, 105:401-409

Li T, Gong CY and Wang T. 2010. RA68 is required for postmeiotic pollen development in Oryza sativa. Plant Molecular Biology, 72:265-277

Li T, Gong CY and Wang T. 2010. The rice light-regulated gene RA68 encodes a novel protein interacting with oxygen-evolving complex PsbO mature protein. Plant Molecular Biology Reporter, 28:136-143

Wang XN, Chen SX, Zhang H, Shi L, Cao FL, Guo LH, Xie YM, Wang T, Yan XF and Dai SJ. 2010. Desiccation tolerance mechanism in resurrection fern-ally selaginella tamariscina revealed by physiological and proteomic analysis. Journal of Proteome Research, 9: 6561–6577 

Xu SB, Li T, Deng ZY, Chong K, Xue YB and Wang T. 2008. Dynamic Proteomic Analysis Reveals a Switch between Central Carbon Metabolism and Alcoholic Fermentation in Rice Filling Grains. Plant Physiology, 148:908-925

Hu YB, Chong K and Wang T. 2008. OsRAF is an ethylene responsive and root abundant factor gene of rice. Plant Growth Regulation, 54:55-61

Hu YB, Zhao LF, Chong K and Wang T. 2008. Overexpression of OsERF1, a novel rice ERF gene, up-regulates ethylene-responsive genes expression besides affects growth and development in Arabidopsis. Journal of Plant Physiology, 165:1717-1725

Dai SJ, Wang T, Yan XF and Chen S. 2007. Proteomics of pollen development and germination. Journal of Proteome Research, 6:4556-4563

Dai XY, Xu YY, Ma QB, Xu WY, Wang T, Xue YB and Chong K. 2007. Overexpression of a R1R2R3 MYB Gene,OsMYB3R-2,Increases Tolerance to Freezing,Drought,and Salt Stress in Transgenic Arabidopsis. Plant Physiology, 143:1739-1751

Deng ZY and Wang T. 2007. OsDMC1 is required for homologous pairing in Oryza sativa. Plant Molecular Biology, 65, 31-42

Tao JY, Zhang LR, Chong K and Wang T. 2007. OsRAD21-3, an orthologue of yeast RAD21, is required forpollen development in Oryza sativa. The Plant Journal, 51919-930

Dai SJ, Chen TT, Chong K, Xue YB, Liu SQ and Wang T. 2007. Proteomic identification of differentially expressed proteins associated with pollen germination and tube growth reveals characteristics of germinated Oryza sativa pollen. Molecular & Cellular Proteomics, 6207-230

Dai SJ, Li L, Chen T., Chong K, Xue YB and Wang T. 2006. Proteomic analyses of Oryza sativa mature pollen reveal novel proteins associated with pollen germination and tube growth. Proteomics, 6:2504-2529

Zhang LR, Tao JY, Wang SX, Chong K and Wang T. 2006. The rice OsRad21-4, an orthologue of yeast Rec8 protein, is required for efficient meiosis. Plant Molecular Biology, 60:533-554

Wang R, Chong K and Wang T. 2006. Divergence in spatial expression patterns and in response to stimuli of tandem-repeat paralogous encoding a novel class of proline-rich proteins in oryza sativa. Journal of Experimental Botany, 57:2887-2897

Zhao LF, Xu SB, Chai TY and Wang T. 2006. OsAP2-1, an AP2-like gene from Oryza sativa, is required for flower development and male fertility. Sexual Plant Reproduction, 19:197-206

Zhang LR, Tao JY and Wang T. 2004. Molecular characterization of OsRAD21-1, a rice homologue of yeast RAD21 essential for mitotic chromosome cohesion. Journal of Experimental Botany, 55: 1149-1152

Ding ZJ, Wu XH and Wang T. 2002. The rice tapetum-specific gene RA39 encodes a type I ribosome-inactivating protein. Sexual Plant Reproduction, 15: 205-212

Ding ZJ, Wang T, Chong K, Bai SN. 2001. Isolation and characterization of OsDMC1, the rice homologue of the yeast DMC1 gene essential for meiosis. Sexual Plant Reproduction, 12: 285-288