姓  名: 漆小泉
职务/职称: 研究员
联系电话: (86)-010-62836671
电子邮件: xqi@ibcas.ac.cn
个人网页: http://www.klpmp.net/ktz_display.asp?ktid=49&ktclass=8
课 题 组: 植物代谢与抗病研究组
漆小泉,男,博士,研究员,博士生导师。

 1984年在华中农业大学获学士学位,1990年在英国伯明翰大学获硕士学位,1998年在荷兰Wageningen大学获博士学位。1998年至2005年在英国John Innes Center做博士后研究,2005年至2006年在英国John Innes CenterSenior Research Associate。现任中国科学院植物研究所植物次生代谢及抗病信号转导创新研究组组长。迄今出版论著1部,发表SCI收录刊物论文30余篇。其中,第一作者和通讯作者论文20余篇。已培养硕士6名,博士12名,博士后5名;正在指导博士生和硕士生12名。

主要研究工作: 

1.植物代谢物的合成及其功能研究 

植物合成并积累种类繁多的代谢物。这些代谢物在植物的生长发育、环境适应中发挥着重要作用;一些特殊的代谢物是重要药物和人体必需的重要营养成分。目前人们对植物代谢物及其合成途径和调控网络还了解甚少。我们以水稻、丹参等为主要研究材料,采用分子生物学以及包括代谢组学在内的分子系统生物分析方法系统地解析植物萜类及其它代谢物的生物合成途径及其调控机理,重点揭示代谢物和及其合成途径的生物学功能。 

2.植物代谢组学及其应用 

植物合成的代谢物有20万至100万种之多,且结构与理化性质差异很大,从而使植物的代谢组学研究更具挑战性。我们使用先进的分析仪器,结合现代组学和系统生物的数据分析手段,进行植物代谢物的定性和定量分析;探索代谢组学在揭示植物科学、农业生产和环境安全等领域的应用前景。 

3.植物持久抗病性的分子机制研究 

作物的持久抗病性在农业生产中具有重要的应用价值,如,小麦条锈病在我国小麦主产区发病严重,危害小麦产量。然而小麦的基因组庞大、复杂,严重阻碍了其功能基因组学的研究。我们利用基因组较小、具有全基因组序列的二穗短柄草为模式植物,解析麦类作物与麦类锈菌的互作机制,为麦类作物的持久抗病品种培育和农业可持续发展提供理论依据。 

研究组在研科研项目: 

1.渤海粮仓高质量绿色发展技术集成与示范,中国科学院,STS项目,(批准号:KFJ-STS-ZDTP-053)(2019.01.01-2020.06.30),资助金额50万元,课题负责人。

2.禾本科作物花粉包被缺失引起的湿敏雄性不育的机制研究,自然科学基金,重点项目,(批准号:31530050)(2016.01.01-2020.12.31,资助资金339.7,项目负责人。

3.规模化高效检测突变体基因新技术研究及其平台建设 农业部,转基因专项,(批准号:2016ZX08009001-006)(2016.01.01-2020.12.31),资助资金164.22万元,任务负责人。

4.水稻功能基因组研究与应用项目 国家任务,国家重点研发计划,(批准号:2016YFD010091-04)(2016.07.01-2020.12.31),资助资金59.74万元,课题参与。

5.天然绿色生物农药的合成生物学与组合合成技术,国家任务,国家重点研发计划,(批准号:2017YFD0201405)(2017.07.01-2020.12.31),资助资金54万元, 子课题主持人。

6.植物性状形成的遗传和进化基础,中国科学院,先导B,(批准号:XDB27010202)(2018.12.03-2023.12.31),资助资金225万元,子课题负责人。

7.长豇豆豆荚氨基酸的定量分析,横向项目,(批准号:2019000052),资助资金13.8万元,项目负责人。

8.蕲艾抗菌消毒产品关键技术研究与开发横向项目,(批准号:2020C025),资助资金30万元,项目负责人。

研究论文(注*为通讯作者): 

2020

Xufan Lian , Xiuli Zhang , Fei Wang , Xiaoning Wang , Zheyong Xue* , Xiaoquan Qi*. 2020. Characterization of a 2,3-oxidosqualene cyclase in the toosendanin biosynthetic pathway of Melia toosendan. Physiologia Plantarum. 170(4):528-536.

Duan LX, Ma AM, Meng XB, Shen GA, Qi XQ*. 2020. QPMASS: A parallel peak alignment and quantification software for the analysis of large-scale gas chromatography-mass spectrometry (GC-MS)-based metabolomics datasets. Journal of Chromatography A, https://doi.org/10.1016/j.chroma.2020.460999

2019

Liu MY, Zhang CJ, Duan LX, Luan QQ, Li JL, Yang AG, Qi XQ*, Ren ZH. 2019. CsMYB60 is a key regulator of flavonols and proanthocyanidans that determine the colour of fruit spines in cucumber. Journal of Experimental Botany, 70: 69-84. (*Corresponding author)

Zhang YC, Chi X, Feng LB, Wu XW, Qi XQ*. 2019. Improvement of multiplex semi-nested PCR system for screening of rare mutations by high-throughput sequencing. BioTechniques, doi:10.2144/btn-2019-0001. (*Corresponding author)

Wang Yajun, Sudeep Subedi, Harmen de Viries, Pieter Doornenbal, Anton Vels, Goetz Hensel, Jochen Kumlehn, Paul A. Johnston, Xiaoquan QiLkram Blilou, Rient E.Niks*, Simon G.Krattinger*. 2019. Orthologous receptor kinases quantitatively affect the host status of barley to leaf rust fungi. Nature Plants, 5:1129-1135. (*Corresponding author)

Wu Hongyu, Xue Xiaodong, Qin Caihua, Xu Yi , Guo Yuyu, Li Xiang, Lv Wei, Li Qinxia, Mao Chuangxue, Li Luzhao, Zhao Suzhen, Qi Xiaoquan, An Hailong. 2019. An Efficient System for Ds Transposon Tagging in Brachypodiurn distachyon. Plant physiology, 180(1):56-65.

2018

Xue ZY, Xu X, Zhou Y, Wang XN, Zhang YC, Liu D, Zhao BB, Duan LX, Qi XQ*. 2018. Deficiency of a triterpene pathway results in humidity-sensitive genic male sterility in rice. Nat. Commun., 9: 604.

Xue ZY, Tan ZW, Huang AC, Zhou Y, Sun JC, Wang XN, Thimmappa RB, Stephenson MJ, Osbourn A*, Qi XQ*. 2018. Identification of key amino acid residues determining product specificity of 2,3-oxidosqualene cyclase in Oryza species. New Phytol., 218: 1076-1088.

2016

Duan LX, Molnár I, Snyder J, Shen GA, Qi XQ. 2016. Discrimination and quantification of true biological signals in LC-MS-based metabolomics analysis. Molecular Plant, doi:10.1016/j.molp.2016.05.009. 

An TY, Cai YL, Zhao SZ, Zhou JH, Song B, Bux H, Qi XQ. 2016. Brachypodium distachyonT-DNA insertion lines: a model pathosystem to study nonhost resistance to wheat stripe rust. Scientific Reports, 6: 25510. 

2015

Cui GH, Duan LX, Jin BL, Qian J, Xue ZY, Shen GA, Snyder J, Song JY, Chen SL, Huang LQ, Peters R, Qi XQ. 2015. Functional divergence of diterpene syntheses in the medicinal plant Salvia miltiorrhiza bunge. Plant Physiology, 169: 1607-1618 

Niks R, Qi XQ, Marcel T. 2015. Quantitative resistance to biotrophic filamentous plant pathogens: Concepts, misconceptions, and mechanisms. Annual Review of Phytopathology, 53: 445-470. 

2014

Xie LX, Tan ZW, Zhou Y, Xu RB, Feng LB, Xing YZ, Qi X. 2014. Identification and fine-mapping of quantitative trait loci for seed vigor in germination and seedling establishment in rice. Journal of Integrative Plant Biology, 56: 749-759. 

Shang Y, Ma YS, Zhou Y, Zhang HM, Duan LX, Chen HM, Zeng JG, Zhou Q, Wang SH, Gu WJ, Liu M, Ren JW, Gu XF, Zhang SP, Wang Y,Yasukawa K, Bouwmeester H, Qi XQ, Zhang ZH,Lucas W, Huang SW. 2014. Biosynthesis, regulation, and domestication of bitterness in cucumber. Science, 346: 1084-1088. 

Chi X, Zhang YC, Xue ZY, Feng LB, Liu HQ, Wang F and Qi XQ. 2014. Discovery of rare mutations in extensively pooled DNA samples using multiple target enrichment. Plant Biotechnology Journal, 12: 709-717. 

2013

Snyder J and Qi XQ*. 2013. BIOSYNTHESIS metal matters. Nat Chem Biol, 9: 295-296   

Sun JC, Xu X, Xue ZY, Snyder JH, Qi X*. 2013. Functional analysis of a rice oxidosqualene cyclase through total gene synthesis. Mol Plant 6: 1726-1729. 

2012

Duan LX, Chen TL, Li M, Chen M, Zhou YQ, Cui GH, Zhao AH, Jia W, Huang LQ and Qi X*. 2012. Use of metabolomics approach to characterize Chinese medicinal material Huangqi. Mol Plant, 5: 376-386 

Cao XH, Zhou JH, Gong XP, Zhao GY, Jia JZ and Qi X*. 2012. Identification and validation of a major quantitative trait locus for slow-rusting resistance to stripe rust in wheat. J Integr Plant Biol, 54: 330344  

Xue ZY, Duan LX, Liu D, Guo J, Ge S, Dicks J, óMáille P, Osbourn A, Qi X*. 2012. Divergent evolution of oxidosqualene cyclases in plants.New Phytol, 193: 1022-1038 

Osbourn A, Papadopoulou KK, Qi X, Field B, Wegel E. 2012. Finding and analyzing plant metabolic gene clusters. Methods Enzymol, 517:113-38 

2010及以前

Wang LJ, Wang YJ, Wang Z, Marcel TC, Niks RE, Qi X*. 2010. The phenotypic expression of QTLs for partial resistance to barley leaf rust during plant development. Theor Appl Genet, 121:857-864 

Wu XD, Qi X*. 2010. Genes encoding hub and bottleneck enzymes of the Arabidopsis metabolic network preferentially retain homeologs through whole genome duplication. BMC Evol Biol, 10:145 

Mugford ST, Qi X, Bakht S, Hill L, Wegel E, Hughes RK, Papadopoulou K, Melton R, Philo M, Sainsbury F, Lomonossoff GP, Deb RA, Goss,RJM, Osbourn A. 2009. A serine carboxypeptidase-like acyltransferase is required for synthesis of antimicrobial compounds and disease resistance in oats. Plant Cell, 21: 2473-2484 

Zhao SZ, Qi X*. 2008. Signaling in plant disease resistance and symbiosis. J Integr Plant Biol, 50(7}: 799-807 

Mylona P, Owatworakit A, Papadopoulou K, Jenner H, Qin B, Findlay K, Hill L, Qi X, Bakht S, Melton R, Osbourn A. 2008. Sad3 and Sad4 are required for saponin biosynthesis and root development in oat. Plant Cell, 20(1): 201-212 

Qi X, Bakht S, Qin B, Leggett M, Hemmings A, Mellon F, Eagles J, Werck- Reichhart D, Schaller H, Lesot A, Melton R, Osbourn A. 2006. A different function for a member of an ancient and highly conserved cytochrome P450 familyFrom essential sterols to plant defense. Proc Natl Acad Sci USA, 103: 18848-18853 

Bakht S, Qi X*. 2005. Ligation mediated rolling-circular amplification (RCA) based approaches to SNP detection. Expert Rev Mol Diagn, 5: 111-116 

Qi X, Pittaway TS, Lindup S, Liu H, Waterman E, Padi FK, Hash CT, Zhu J, Gale MD, Devos KM. 2004. An integrated genetic map and a new set of SSR markers for pearl millet Pennisetum glaucum. Theor Appl Genet, 109: 1485-1493 

Qi X, Bakht S, Leggett M, Maxwell C, Melton R, Osbourn A. 2004. Clustered genes for secondary metabolism in oat-Implications for the evolution of metabolic diversity in plants. Proc Natl Acad Sci USA, 101: 8233-8238 

Osbourn AE, Qi X, Townsend B, Qin B. 2003. Dissecting plant secondary metabolism constitutive chemical defenses in cereals. New Phytol, 159: 101-108 

Haralampidis K, Bryan G, Qi X, Papadopoulou K, Bakht S, Melton R, Osbourn A. 2001. A new class of oxidosqualene cyclases directs synthesis of antimicrobial phytoprotectants in monocots. Proc Natl Acad Sci USA, 98: 13431-13436 

Qi X*, Bakht S, Devos KM, Gale MD, Osbourn A. 2001. L-RCA (ligation-rolling circle amplification): a general method for genotyping of single nucleotide polymorphisms (SNPs). Nucleic Acid Res, 29: e116 

Qi X*, Lindup S, Pittaway TS, Allouis S, Gale MD, Devos KM. 2001. Development of simple sequence repeat (SSR) markers from bacterial artificial chromosomes (BACs) without sub-cloning. BioTechniques, 31: 355-362 

Allouis S, Qi X, Lindup S, Gale MD, Devos KM. 2001. Construction of a pearl millet BAC library using pBAC/SACB1a positive selection vectorTheor Appl Genet, 102: 1200-1205 

Qi X*, Fufa F, Sijtsma D, Niks RE, Lindhout P, Stam P. 2000. The evidence for abundance of QTLs for partial resistance to Puccinia hordei on the barley genome. Mol Breed, 6: 1-9 

Qi X*, Jiang GL, Chen WQ, Niks RE, Stam P, Lindhout P. 1999. Isolate-specific QTLs for partial resistance to Puccinia hordei in barley. Theor Appl Genet, 99: 877-884 

Qi X*, Niks RE, Stam P, Lindhout P. 1998. Identification of QTLs for partial resistance to leaf rust (Puccinia hordei) in barley. Theor Appl Genet, 96: 1205-1215 

Qi X*, Stam P, Lindhout P. 1998. Use of locus-specific AFLP markers to construct a high-density molecular map in barley. Theor Appl Genet, 96: 376-384 

Schut JW, Qi X, Stam P. 1997. Relationship between AFLP based genetic similarities and coefficients of co-ancestry in barleyTheor Appl Genet ,95: 1161-1168 

Qi X*, Lindhout P. 1997. Development of AFLP markers in barleyMol Gen Genet, 254: 330-336 

Qi X*, Stam P, Lindhout P. 1996. Comparison and integration of four barley genetic maps. Genome, 39: 379-394 

著作专著: 

[1]  漆小泉,王玉兰,陈晓亚主编,2011。植物代谢组学-方法与应用。北京:化学工业出版社。(中文) 

专利: 

[1]  漆小泉,薛哲勇,段礼新. 帕克醇合成相关蛋白及其编码基因与应用: 中国, ZL200810117225.X. 2011-03-30.  

[2] 漆小泉,薛哲勇,段礼新. 异山柑子萜醇合成相关蛋白及其编码基因与应用: 中国, ZL200810117226.4. 2011-04-20. 

[3] 漆小泉,薛哲勇,刘丹,段礼新. 一种新的三萜合成酶的功能及应用;中国,ZL2011102479987.7. 2013-08-14 

[4] 漆小泉,曹晓花. 一种多重检测基因组DNA多态性的方法及其专用探针:中国,ZL201110121242.2. 2013-10-23. 

[5] 薛哲勇,漆小泉,孙俊聪,段礼新. 一种优化三萜合酶编码基因及其应用:中国,ZL201310002085.2. 2014-03-26