姓  名: 王柏臣
职务/职称: 研究员
联系电话: 86-10-62836884
电子邮件: wangbc@ibcas.ac.cn
个人网页: http://www.klpbcas.com/yjdw/ktzz/wbc/
课 题 组: 光合碳代谢研究组
王柏臣,男,博士,研究员,博士生导师。

2002年在哈尔滨师范大学获得硕士学位,2006年在北京大学获博士学位。20069月至20116月在东北林业大学担任教教授从事教学和科研工作。以通讯作者和第一作者发表SCI收录论文10余篇。已培养硕士和博士15名;在读博士生和硕士生10名。

主要研究工作:

1 植物光合碳代谢研究:

C4植物玉米和C3植物拟南芥和杨树为研究材料,利用分子生物学,生物化学、细胞学和遗传学方法研究C4植物高效固碳的分子生物学机理以及C4途径关键酶酶转录、翻译、翻译后修饰以及酶活活性的调控机制。

主持和参加的科研项目:

1作物高光效利用的分子机制,中国科学院战略性先导科技专项;子课题编号:XDA240102032019.11-2024.12)资助额度:1084万元,子课题负责人。

2)杨树和苜蓿等重要林草植物基因编辑技术的建立,横向课题;项目编号:2019C092,(2019.11-2023.10)资助额度:500万元,主持人。

3)盐碱地草业发展关键技术集成与示范,中国科学院科技服务网络计划;编号:2016ZX08009003-005-001,(2019.1-2020.6)资助额度:40万元,课题负责人。

4)高光效优质牧草品种培育和扩繁技术研发,横向课题;项目编号:2019C004,(2019.1-2023.12)资助额度:100万元,主持人。

5)玉米光合固碳途径关键酶PPDK调节蛋白PDRP的双功能调控,国家自然科学基金面上项目;编号:31770262,(2018.1-2021.12)资助额度:60万元,主持人。

6)玉米产量、品质性状的功能基因组与调控网络,国家重点基础研究发展计划;课题编号:2016YFD0101003,(2016.7-2021.12)资助额度:60万元,子课题负责人。

7)重要性状基因克隆及功能验证,转基因生物新品种培育重大专项;编号:2016ZX08009003-005-001,(2016.1-2020.12)资助额度:135万元,任务负责人。

8)玉米PEPCK-C4光合碳代谢途径的调控机制研究,国家自然科学基金面上项目;编号:31471506,(2015.1-2018.12)资助额度:100万元,主持人。

9)玉米矮杆基因及其调控网络的发掘,国家自然科学基金重大研究计划培育项目;编号:91435109,(2015.1-2017.12)资助额度:100万元,主持人。

10)玉米水分高效利用分子模块解析,中国科学院战略性先导科技专项;子课题编号:XDA080102062013.8-2018.7)资助额度:3089万元,子课题负责人。

11)杨树抗旱光合关键基因的鉴定及分子育种技术研究,863项目,批准号:2013AA1027012013.1-2017.12)资助额度:133万元,子课题负责人。

12)光高效玉米种植模式增产机理研究,中国科学院科技服务网络计划,项目编号:KFJ-EW-STS-0662014.5-2016.5)资助额度:79万元,课题负责人。

13)玉米高光效QTL克隆及分子机理研究,863项目,批准号:2012AA10A3002012.1-2015.12)资助额度:66万元,子课题负责人。

14)玉米高光效分子机理研究,中国科学院知识创新工程项目; 批准号:O2819G1001,(2011.1-2013.12)资助额度:200万元,主持人。

15)杨树引入C4光合固碳途径关键酶的分子基础研究,国家自然基金重点项目; 批准号:31030017,(2011.1-2014.12)资助额度:204万元,主持人。

研究论文(注*为通讯作者):

2020

(1). Yan Z, Shen Z, Chao Q, Kong L, Gao ZF, Li QW, Zheng HY, Zhao CF, Lu CM, Wang YW*, Wang BC*. 2020. Genome-wideTranscriptome and Proteome Profiles Indicate an Active Role of Alternative Splicing During De-etiolation of Maize Seedlings. Planta, 252:60.

(2). Gao ZF, Shen Z, Chao Q, Yan Z, Ge XL, Lu TC, Zheng HY, Qian CR*, Wang BC*. 2020. Large-scale Proteomic and Phosphoproteomic Analysis of Maize Seedling Leaves During De-etiolation. Genom Proteom Bioinf, 18: 6.

(3). Yan Z, Shen Z, Gao ZF, Chao Q, Qian CR, Zheng HY, Wang BC*. 2020. A Comprehensive Analysis of the Lysine Acetylome Reveals Diverse Functions of Acetylated Proteins During De-etiolation in Zea mays. J. Plant Physiol., 248:153-158.

2019

(4). Chao Q, Gao ZF, Zhang D, Zhao BG, Dong FQ, Fu CX, Liu LJ, Wang BC*. 2019. The developmental dynamics of the Populus stem transcriptome. Plant Biotechnol. J., 17: 206-219.

(5). Wang YF,  Chao Q, Li Z, Lu TC, Zheng HY, Zhao CF, Shen Z, Li XH*, Wang BC*. 2019. Large-scale Identification and Time-course Quantification of Ubiquitylation Events During Maize Seedling De-etiolation. Genom Proteom Bioinf, 17: 603-622.

2017

(6). Li Y, Dong XM., Jin F, Shen Z, Chao Q, Wang BC. 2017. Histone acetylation modifications affect tissue-dependent expression of poplar homologs of C4 photosynthetic enzyme genes. Front Plant Sci., 8: 950.

(7).      Li Y, Jin F, Chao Q, Wang BC*. 2017. Proteomics Analysis Reveals the Molecular Mechanism Underlying the Transition From Primary to Secondary Growth of Poplar. J. Plant Physiol., 213: 1-15.

(8). Shen Z, Dong XM, Gao ZF, Chao Q, Wang BC. 2017. Phylogenic and phosphorylation regulation difference of phosphoenolpyruvate carboxykinase of C3 and C4 plants. J. Plant Physiol., 213: 16-22.

(9). Bu TT, Shen J, Chao Q, Shen Z, Yan Z, Zheng HY, Wang BC. 2017. Dynamic N-glycoproteome analysis of maize seedling leaves during de-etiolation using Concanavalin A lectin affinity chromatography and a nano-LC–MS/MS-based iTRAQ approach. Plant Cell Rep., 36: 1943-1958.

2016

(10).   Jiang Lun, Chen Yibo, Zheng Jiangge, Chen Zhenhang, Liu Yujie, Tao Ye, Wu Wei, Chen Zhongzhou, Wang Baichen*. 2016. Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein. Plant Physiology, 170: 732-741.

(11).   Chao Qing, Gao Zhifang, Wang Yuefeng, Li Zhe, Huang Xiahe, Wang Yingchun, Mei Yingchang, Zhao Biligegaowa, Li Liang, Jiang Yubo, and Wang Baichen*. 2016. The proteome and phosphoproteome of maize pollen uncovers fertility candidate proteins. Plant Molecular Biology, 91: 287-304.

(12).   Dong Xiumei, Li Yuan, Chao Qing, Shen Jie, Gong Xiujie, Zhao Biligengaowa, Wang Baichen*. 2016. Analysis of gene expression and histone modification between C4 and non-C4 homologous genes of PPDK and PCK in maize. Photosynthesis Research, 129: 71-83.

(13).   Yibo Chen, Dan Wang, Xuanling Ge, Biligengaowa Zhao, Xuchu Wang and Baichen Wang*. 2016. Comparative Proteomics of Leaves Found at Different Stem Positions of Maize Seedlings. Journal of Plant Physiology, 198: 116-28.

(14).   Deli Ning, Kehui Liu, Changcai Liu, Jinwen Liu, Chunrong Qian, Yang Yu, Yuefeng Wang, Yingchun Wang and Baichen Wang*. 2016. Large-scale Comparative Phosphoprotein Analysis of Maize Seedling Leaves During Greening. Planta, 243: 501-507.

2015

(15).   Ning Dengli, Liu Kehui, Liu Changchai, Liu Jinwen, Qian chunrong, Yu Yang, Wang Yuefeng, Wang Yingchun, Wang Baichen*. 2015. Large-scale comparative phosphoprotein analysis of maize seedling leaves during greening. Planta, 1-17.

(16).   Jiang Lun, Chen Yibo, Zheng jiangge, Chen Zhenhang, Liu Yujie, Tao Ye, Wu Wei, Chen Zhongzhou, Wang Baichen*. 2015. Structural basis of reversible phosphorylation by maize pyruvate orthophosphate dikinase regulatory protein (PDRP). Plant Physiology, 170(2), 732.

2014

(17).   Meng Lingbo, Chen Yibo, Lu Tiancong, Wang Yuefeng, Qian Chunrong, Yu Yang, Ge Xuangliang, Li Xiaohui, Wang Baichen*. 2014. A systematic proteomic analysis of NaCl-stressed germinating maize seeds. Molecular Biology Reports, 41(5): 3431-3443.

2011

(18).   Bi YD, Wang HX, Lu TC, Li XH, Shen Z, Chen YB, Wang BC*. 2011. Large-scale analysis of phosphorylated proteins in maize leaf. Planta. 233:383-392 (SCI IF: 3.35).

(19).   Liu XY, Wu YD, Shen ZY, Shen Z, Li HH, Yu XM, Yan XF, Guo CH, Wang BC*. 2011. Shotgun proteomics analysis on maize chloroplast thylakoid membrane. Front Biosci. 3:250-5 (SCI IF: 3.736).

(20).   Bi YD, Wei ZG, Shen Z, Lu TC, Cheng YX, Wang BC*, Yang CP. 2011. Comparative temporal analyses of the Pinus sylvestris L. var. mongolica litv. apical bud proteome from dormancy to growth. Mol Biol Rep. 38721-729 (SCI IF: 2.03).

2010及以前

(21).   Liu CC, Lu TC, Li HH, Wang HX, Liu GF, Ma L, Yang CP, Wang BC*. 2010. Phosphoproteomic identification and phylogenetic analysis of ribosomal P-proteins in Populus dormant terminal buds. Planta 231571-581 (SCI IF: 3.35).

(22).   Ni RJ, Shen Z, Yang CP, Wu YD, Bi YD, Wang BC*. 2010. Identification of low abundance polyA-binding proteins in Arabidopsis chloroplast using polyA-affinity column. Mol Biol Rep. 37637-641 (SCI IF: 2.03).

(23).   Shen Z, Li P, Ni RJ, Mark R, Yang CP, Liu GF, Ma W, Liu GJ, Ma L, Li SJ, Wei ZG, Wang HX, Wang BC*. 2009. Label-free quantitative proteomics analysis of etiolated maize seedling leaves during greening. Mol. Cellular Proteomics 8: 2443-2460 (SCI IF: 8.84).

(24).   Zhu H, Bi YD, Yu LJ, Guo DD, Wang BC*. 2009. Comparative proteomic analysis of apomictic monosomic addition line of Beta corolliflora and Beta vulgaris L. in sugar beet. Mol Biol Rep. 36:2093-8 (SCI IF: 2.03).

(25).   Lu TC, Meng LB, Yang CP, Liu GF, Liu GJ, Ma W, Wang BC*. 2008. A shotgun phosphoproteomics analysis of embryos in germinated maize seeds. Planta, 228: 1029–1041 (SCI IF: 3.35).

(26).   Wu FZ, Lu TC, Shen Z, Wang BC*, Wang HX. 2008. N-Terminal acetylation of two major latex proteins from Arabidopsis thaliana using electrospray ionization tandem mass spectrometry. Plant Mol. Biol. Rep. 26: 88–97. (SCI IF: 0.74).

(27).   Wang BC, Pan YH, Meng DZ, Zhu YX. 2006. Identification and Quantitative Analysis of Significantly Accumulated Proteins During the Arabidopsis Seedling De-etiolation Process. J. Integr. Plant. BioL. 48: 104-113. (SCI IF:0.86).

(28).   Wang BC, Wang HX, Feng JX, Meng DZ, Qu LJ, Zhu YX. 2006. Post-translational modifications, but not transcriptional regulation, of major chloroplast RNA-binding proteins are related to Arabidopsis seedling development. Proteomics, 6: 2555-2563 (SCI IF: 5.76).